WormBase Tree Display for Expr_pattern: Expr2418
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| Expr2418 | Expression_of | Gene | WBGene00006750 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006750 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| Anatomy_term | WBbt:0005829 | Certain | ||
| WBbt:0006749 | Certain | |||
| WBbt:0006750 | Certain | |||
| GO_term | GO:0008021 | |||
| Subcellular_localization | Double labeling with antibodies directed against RAB-3, a synaptic vesicle protein, revealed that Rim was much more tightly clustered than synaptic vesicle markers. In the head of the worm, where individual synaptic varicosities of SAB neurons can be examined, one or two Rim puncta were localized in the middle of each RAB-3 staining synaptic varicosity. Localized Rim protein was undetectable in cell bodies. | |||
| Type | Antibody | Polyclonal rabbit antibody. Antibodies were raised against a His6-tagged fusion to amino acids 1-142 of UNC-10. The fusion protein was purified on Ni-NTA agarose in 8M urea, and renatured by dialysis against phosphate buffered saline. Rabbit 5431 antiserum was used at 1:12,500 for immunohistochemistry. | ||
| Pattern | Rim staining was restricted to discrete puncta in synapse-rich regions of the nervous system including the nerve ring, the ventral nerve cord and the dorsal nerve cord. Localized Rim protein was observed in all larval stages. | |||
| Remark | In unc-10(md1117) animals that lack the entire Rim coding region, no immunoreactivity was observed with the anti-Rim antibody, although RAB-3 was normally localized. | |||
| Reference | WBPaper00004891 | |||
| Antibody_info | WBAntibody00000412 |
