WormBase Tree Display for Expr_pattern: Expr2412
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| Expr2412 | Expression_of | Gene | WBGene00000802 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000802 | |||
| Expression_data | Anatomy_term | WBbt:0003681 | Certain | |
| WBbt:0005792 | Certain | |||
| WBbt:0005812 | Certain | |||
| WBbt:0005813 | Certain | |||
| WBbt:0006751 | Certain | |||
| WBbt:0006798 | Certain | |||
| GO_term | GO:0005737 | |||
| Subcellular_localization | CRT-1 was detected in the cytoplasm, presumably from the endoplasmic reticulum network. | |||
| Type | Antibody | Polyclonal rabbit antibody. The full-length crt-1 cDNA was subcloned into pGEX-4T1 (Amersham Pharmacia Biotech) and overexpressed as glutathione S-transferase (GST) fusion protein. The fusion protein was purified on Glutathione-Sepharose (Amersham Pharmacia Biotech) and used to immunize rabbits. Whole-mount immunostaining with anti-CRT-1 antibodies in a mixed stage wild-type population. | ||
| Pattern | CRT-1 was detected in the cytoplasm, presumably from the endoplasmic reticulum network, of the early embryo (~100-cell stage) and a strong intestinal staining was observed in both larval and adult worms. Signals were also observed in the terminal bulb region of the pharynx and in excretory cells in the head. Faint but specific staining was also observed in head neurons and body-wall muscle cells. | |||
| Picture | WBPicture0000008696 | |||
| WBPicture0000008698 | ||||
| Reference | WBPaper00004858 | |||
| Antibody_info | WBAntibody00000408 |
