WormBase Tree Display for Expr_pattern: Expr2284
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| Expr2284 | Expression_of | Gene | WBGene00004955 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004955 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| WBls:0000041 | |||
| GO_term | GO:0005813 | ||
| Subcellular_localization | SPD-5 was first detected in early postmeiotic one-cell stage embryos, prior to pronuclear migration but after the sperm-donated centriole had duplicated to produce two detectable foci of SPD-5 staining. These two foci greatly increased in size prior to and during mitosis. In metaphase, SPD-5 was evenly distributed throughout centrosomes. In late anaphase or telophase, SPD-5 surrounded a central core devoid of both MTs and SPD-5. | ||
| Type | Antibody | Polyclonal antibodies specific for SPD-5 to stain fixed embryos | |
| Pattern | SPD-5 was detected at centrosomes throughout embryogenesis and during postembryonic meiotic divisions that produce sperm in male nematodes. Paternal requirement for SPD-5 was not detected after raising homozygous spd-5(or213ts) males at the restrictive temperature throughout larval development. SPD-5 was not detectable in mature spermatids. | ||
| Picture | WBPicture0000009911 | ||
| Remark | Two different antisera that recognize the same SPD-5 peptide, and one that recognizes a different SPD-5 peptide, gave identical staining patterns, and no foci of SPD-5 protein were detected in fixed spd-5(RNAi) or spd-5(or213ts) mutants during the first attempt at mitosis. | ||
| Reference | WBPaper00005565 | ||
| Antibody_info | WBAntibody00000572 | ||
| WBAntibody00000573 |
