WormBase Tree Display for Expr_pattern: Expr2283

Expr2283 Expression_of: Gene: WBGene00006745
    Reflects_endogenous_expression_of: WBGene00006745
  Expression_data: Life_stage: WBls:0000002
    Anatomy_term: WBbt:0004506 Certain
      WBbt:0004520 Certain
      WBbt:0005409 Certain
      WBbt:0005775 Certain
      WBbt:0005829 Certain
      WBbt:0006750 Certain
    GO_term: GO:0030424
      GO:0043025
  Subcellular_localization: UNC-5::GFP was punctate in all tissues in which it was detected. In neurons, punctate expression is distributed in cell bodies and along axons. Punctate expression was also observed in the veil-like extensions of the following portion of the DTC, which wraps the distal gonad arm. However, UNC-5::GFP appears more abundant around the nucleus at the leading end of the cell and frequently appears most concentrated in the narrow region between the nucleus and the leading edge membrane of the DTC.
  Type: Reporter_gene
  Pattern: The tagged versions of UNC-5 appear to be expressed in all of the same cell types and with the same timing as previously described for unc-5 transcriptional reporters (see Expr962). Expression of UNC-5::GFP was observed in motor neurons during the comma stage of embryogenesis when axon outgrowth begins, in the ventral nerve cord, the dorsal nerve cord, the commissural motor axons running between the two cords, the excretory canal, and in the DTCs as they migrate toward and then along the dorsal side of the animal.
    Up-regulation of unc-5 transcriptional reporters was previously shown to be coincident with the beginning of the phase 2 (dorsal to ventral) migration of the DTCs (see Expr962). DTC expression appears slightly delayed when GFP reporters rather than lacZ reporters are used, possibly because of the time it takes for the GFP fluorophore to mature.
  Reference: WBPaper00005582
  Transgene: WBTransgene00027981