WormBase Tree Display for Expr_pattern: Expr1995
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| Expr1995 | Expression_of | Gene | WBGene00000554 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000554 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000018 | ||||
| WBls:0000019 | ||||
| WBls:0000020 | ||||
| WBls:0000017 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0005319 | Certain | ||
| WBbt:0005753 | Certain | |||
| WBbt:0005813 | Certain | |||
| WBbt:0005821 | Certain | |||
| WBbt:0006751 | Partial | |||
| WBbt:0006759 | Partial | |||
| WBbt:0006794 | Certain | |||
| GO_term | GO:0005737 | |||
| Subcellular_localization | Scattered and distinct cytoplasmic signals of CNB-1 was observed surrounding the cellularized spermatids. | |||
| Wildtype male sperm was examined and immunostained with antiCNB-1 antibody. As expected, robust staining was observed in the wild-type sperm and the staining was distinctly cytoplasmic. | ||||
| Type | Antibody | Polyclonal antibody against CNB-1 was generated, and western blots with total protein extracts from wild-type worms detected a single band around 16 kDa for CNB-1. | ||
| Pattern | CNB-1 localization was also confirmed in the spermatheca by immunostaining isolated gonads. | |||
| CNB-1 was localized at the subcellular level in specific tissues of wild-type animals by immunogold electron microscopy (EM). Signals of CNB-1 were observed in the seam cells of the lateral hypodermis of wild-type hermaphrodites consistent with the immunostaining data. Additionally, the male gonad also expressed CNB-1. This was evident from the scattered and distinct cytoplasmic signals of CNB-1 surrounding the cellularized spermatids. | ||||
| Similar localization to GFP expression patterns and additionally showed localization in hypodermal seam cells. See Expr1993 for cnb-1::gfp expression patterns. | ||||
| Picture | WBPicture0000008711 | |||
| WBPicture0000008712 | ||||
| Reference | WBPaper00005483 | |||
| Antibody_info | WBAntibody00000561 |
