WormBase Tree Display for Expr_pattern: Expr1976
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| Expr1976 | Expression_of | Gene | WBGene00003975 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003975 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0003675 | Certain | ||
| WBbt:0003679 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0006748 | Certain | |||
| GO_term | GO:0005783 | |||
| GO:0005794 | ||||
| Subcellular_localization | In later stages, most cells show strong perinuclear localization of GFP and a nonuniform cytosolic signal, which likely represents localization to intracellular membranes, such as endoplasmic reticulum (ER) and Golgi. | |||
| Type | Reporter_gene | |||
| Pattern | The N-terminal and loop pen-2::GFP fusions gave strong GFP fluorescence in most somatic tissues beginning at the 100-cell stage of development, including neurons, muscle, intestine, and the developing vulva. pen-2::GFP fluorescence was not detectable in early embryos, even though the pen-2::GFP transgenes rescue early pen-2 function. | |||
| Picture | WBPicture0000009910 | |||
| Remark | Transgenes expressing pen-2 with GFP inserted at the N terminus or within the predicted loop between the two transmembrane domains efficiently rescue the Egl and Mel pheno types of pen-2(ep220), indicating that both constructs encode functional PEN-2 proteins. In contrast, GFP fused to the C terminus induces a dominant-negative Mel/Aph phenotype in an otherwise wild-type background, similar to the pen-2 loss of function phenotype, indicating an important function for pen-2 C-terminal sequences. | |||
| Reference | WBPaper00005374 | |||
| Transgene | WBTransgene00027786 |
