WormBase Tree Display for Expr_pattern: Expr1976
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Expr1976 | Expression_of (2) | |
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Expression_data (3) | ||
Subcellular_localization | In later stages, most cells show strong perinuclear localization of GFP and a nonuniform cytosolic signal, which likely represents localization to intracellular membranes, such as endoplasmic reticulum (ER) and Golgi. | |
Type | Reporter_gene | |
Pattern | The N-terminal and loop pen-2::GFP fusions gave strong GFP fluorescence in most somatic tissues beginning at the 100-cell stage of development, including neurons, muscle, intestine, and the developing vulva. pen-2::GFP fluorescence was not detectable in early embryos, even though the pen-2::GFP transgenes rescue early pen-2 function. | |
Picture | WBPicture0000009910 | |
Remark | Transgenes expressing pen-2 with GFP inserted at the N terminus or within the predicted loop between the two transmembrane domains efficiently rescue the Egl and Mel pheno types of pen-2(ep220), indicating that both constructs encode functional PEN-2 proteins. In contrast, GFP fused to the C terminus induces a dominant-negative Mel/Aph phenotype in an otherwise wild-type background, similar to the pen-2 loss of function phenotype, indicating an important function for pen-2 C-terminal sequences. | |
Reference | WBPaper00005374 | |
Transgene | WBTransgene00027786 |