WormBase Tree Display for Expr_pattern: Expr1885
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| Expr1885 | Expression_of | Gene | WBGene00004889 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004889 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| WBls:0000015 | |||
| WBls:0000021 | |||
| WBls:0000010 | |||
| WBls:0000013 | |||
| Anatomy_term (36) | |||
| GO_term | GO:0030424 | ||
| GO:0043025 | |||
| Type | Reporter_gene | ||
| Pattern | In animals transgenic for the smp-1::lacZ reporter constructs, beta-galactosidase is first detected in epidermal cells at the beginning of morphogenesis 200 minutes after fertilization. GFP fluorescence from smp-1::gfp expression is initially observed at approximately the 50 cell stage in the E lineage. Later, in larvae and adults, GFP can be seen in all body wall, vulval, uterine and enteric muscles, as well as male-specific muscles of the tail and copulatory system. In adults, smp-1::gfp is expressed in the tail tip (hyp 10), in ray 6 and in the spicules of the adult male. Approximately 10 sensory neuron support cells in the head with dendrites extending to the tip of the head, also express the smp-1 GFP and beta-galactosidase transcriptional reporters. GFP fluorescence is observed in several individual cells, including an interneuron (tentatively identified as AVL), the excretory channel, the distal tip cells (DTCs) throughout their migration, somatic cells of the gonad, and epidermal cells hyp 4 (and possibly hyp 3) and hyp 10. In the adult, expression was observed in the fused seam cell syncytium comprising the lateral epidermis. Although ray 6 expresses in the adult male tail, it is difficult to determine whether the ray 6 precursors or any other ray or SET precursors or progeny express smp-1::gfp during the L3 and L4 stages of development when the male tail is forming. This is because GFP fluorescence in the male sex-specific muscles is so bright at this stage as to obscure what may be faint expression of other cells in the male tail. | ||
| Picture | WBPicture0000007499 | ||
| WBPicture0000007502 | |||
| Reference | WBPaper00005258 | ||
| Transgene | WBTransgene00027735 |
