WormBase Tree Display for Expr_pattern: Expr1849
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Expr1849 | Expression_of | Gene | WBGene00003865 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003865 | ||
Expression_data (2) | |||
Type | Western | ||
Pattern | Assay 1: A developmental Western blot using synchronized populations indicates that OMA-2 was expressed only in the adult with very little, if any, expression detectable in L1 through L4 larval stages. | ||
Assay 2: Extracts were prepared from three temperature-sensitive mutant strains that are defective in different aspects of germline development. At the permissive temperature (15 Centigrades) in all three strains, OMA-2 was expressed. At the nonpermissive temperature (25 Centigrades), glp-4(bn2) mutant worms do not produce a germline and do not express OMA-2. fem-3(e2006) mutant worms produce only oocytes and no sperm at 25 C and express OMA-1. Semidominant mutants fem-3(q20sd) at 25 C produce only sperm and no oocytes and do not express OMA-2. Thus, OMA-2 protein is expressed primarily in adult oocytes but not in sperm, consistent with the functional assay. | |||
Picture | WBPicture0000009903 | ||
Reference | WBPaper00005198 | ||
Antibody_info | WBAntibody00000487 |