WormBase Tree Display for Expr_pattern: Expr1848
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| Expr1848 | Expression_of | Gene | WBGene00003864 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003864 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| Anatomy_term | WBbt:0006797 | Certain | ||
| Type | Western | |||
| Pattern | Assay 1: A developmental Western blot using synchronized populations indicates that OMA-1 was expressed only in the adult with very little, if any, expression detectable in L1 through L4 larval stages. | |||
| Assay 2: Extracts were prepared from three temperature-sensitive mutant strains that are defective in different aspects of germline development. At the permissive temperature (15 Centigrades) in all three strains, OMA-1 was expressed. At the nonpermissive temperature (25 Centigrades), glp-4(bn2) mutant worms do not produce a germline and do not express OMA-1. fem-3(e2006) mutant worms produce only oocytes and no sperm at 25 C and express OMA-1. Semidominant mutants fem-3(q20sd) at 25 C produce only sperm and no oocytes and do not express OMA-1. Thus, OMA-1 protein is expressed primarily in adult oocytes but not in sperm, consistent with the functional assay. | ||||
| Picture | WBPicture0000009903 | |||
| Reference | WBPaper00005198 | |||
| Antibody_info | WBAntibody00000486 |
