WormBase Tree Display for Expr_pattern: Expr1816

Expr1816 Expression_of: Gene: WBGene00004736
    Reflects_endogenous_expression_of: WBGene00004736
  Expression_data: Life_stage: WBls:0000024
      WBls:0000003
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
    Anatomy_term (14)
    GO_term: GO:0005783
      GO:0005635
      GO:0031410
      GO:0016529
      GO:0042175
  Subcellular_localization: Fluorescence from Psca-1::GFP was evenly distributed over the cytosol. However, both CeSERCAa::GFP and CeSERCAb::GFP showed specific subcellular localization. Both fusion proteins were found in a tubular meshwork that was most distinct in the body wall muscle cells. This staining was continuous with staining at or near the dense bodies, structures functionally analogous to vertebrate Z-lines. In body wall muscle cells and other cells, staining was also often localized in internal vesicles and membrane-like structures, including the nuclear envelope.
  Type: Reporter_gene
  Pattern: All three reporter constructs were expressed in all major contractile tissues, starting during embryogenesis and continuing until adulthood. Fluorescence was particularly pronounced in striated muscle (body wall muscles used for locomotion), non-striated muscle (pharyngeal muscles used for pharyngeal pumping, vulval and uterine muscles used for egg laying, the sphincter muscle and anal depressor used for defecation), and in myoepithelial cells (gonadal sheath cells used for ovulation). Furthermore, all three constructs showed expression in the intestine. Some cells expressed CeSERCAb::GFP but not CeSERCAa::GFP. These include the somatic cells of the spermatheca and the excretory canal and the uterine sheath cells. No cells were found that expressed CeSERCAa::GFP but not CeSERCAb::GFP.
  Picture: WBPicture0000008169
    WBPicture0000008170
  Reference: WBPaper00004979
  Transgene: WBTransgene00027703