WormBase Tree Display for Expr_pattern: Expr1680

Expr1680 Expression_of: Gene: WBGene00002978
    Reflects_endogenous_expression_of: WBGene00002978
  Expression_data: Life_stage: WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
    Anatomy_term: WBbt:0003681 Certain
      WBbt:0003721 Certain
      WBbt:0003737 Certain
      WBbt:0003739 Certain
      WBbt:0003740 Certain
      WBbt:0003744 Certain
      WBbt:0005772 Certain
      WBbt:0005784 Certain
      WBbt:0005792 Certain
  Type: Reporter_gene
  Pattern: The constructs pTMZIII4135 and pTMZIII1743 permanently induced beta-galactosidase expression in pharyngeal muscles and intestinal cells of the worm from L4 stage. Pharyngeal expression was observed in m1, m3, m4, m5 and m7 muscle cells. Between the L2 and L3 stages, both constructs induced expression in tissues corresponding to germ-line tissue of the gonadal primordium: one anterior and one posterior. At this stage, intestinal expression was restricted to the most posterior part of the worm. The expression in the germ-line tissue was eliminated by L4 stage. The beta-galactosidase expression of CeTMIII isoform was stage specific. In about 95 % of L1 worms, expression was observed in the pharynx only; between L2 and L3 stages, the expression extended further to germ-line tissue and intestinal cells. Permanent expressions in pharynx and intestines were evident from L4 stage and continued to adulthood. The pharyngeal staining was much stronger than those of germ-line tissue and intestines. Unlike CeTMIV isoform, embryonic expression of CeTMIII isoform was absent and the expression intensity of intestinal cells did not decrease with stage.
  Picture: WBPicture0000010188
  Remark: Reporter gene fusion type not specified.
    This Expr_pattern is about CeTMIII, an isoform of tmy-1 transcription.
  Reference: WBPaper00004944
  Transgene: WBTransgene00027608