WormBase Tree Display for Expr_pattern: Expr1614

Expr1614 Expression_of: Gene: WBGene00000395
    Reflects_endogenous_expression_of: WBGene00000395
  Expression_data: Life_stage: WBls:0000002
    Anatomy_term (49)
  Type: Reporter_gene
  Pattern: At the beginning of embryonic morphogenesis, all ten pairs of lateral epidermal cells, the seam cells, express cdh-3::lacZ. cdh-3::GFP expression in these cells is observed at hatching and throughout subsequent postembryonic development. During the last larval stage (L4) the 15 pairs of seam cells generated during larval development fuse to form two continuous lateral syncytia, surrounded by hyp7. cdh-3::GFP expression correlates with seam cell identity during these postembryonic divisions; it is not observed in daughters that fuse with hyp7 or adopt other fates. In embryos undergoing morphogenesis, lacZ is expressed in a single large nucleus, whose size and position is consistent with that of the excretory cell. This identification is reinforced by the observation that in many newly hatched L1 larvae, low levels of GFP expression are visible in the excretory cell. This expression of cdh-3-reporter genes was observed only during late embryogenesis and in some newly hatched L1s (the latter may be due to perdurance of the GFP fusion protein), but not at later stages. Several other cells expressed the cdh-3 reporter constructs during embryonic morphogenesis; as in the seam cells this expression continued upon hatching. In the tail two cells, hyp10 and hyp11, show strong GFP expression that persists only during the first larval stage. Strong expression was observed in cells that form interfacial epithelia between the intestinal epithelium and the epidermis. Two cells that form part of the rectal epithelium, designated F and U, express cdh-3::GFP during embryonic morphogenesis and throughout larval development. In the anterior of embryos undergoing morphogenesis there were several cells expressing cdh-3::lacZ, and in larvae and adults, GFP expression is seen in nine cell bodies located just anterior to the first bulb of the pharynx. Processes extend anteriorly from these cell bodies and terminate at the level of the buccal capsule. Based upon the location of the cell bodies and the morphology of the processes, these cells were identified as the anterior and posterior arcade cells.
    Expression pattern of the pJP#38 construct in males indicated expression in the male tail and several male-specific neurons, however the expression pattern is complicated and the cell identity were not determined.
    cdh-3::GFP expressed in the developing hermaphrodite vulva. GFP first expressed in the anchor cell in L3 larvae. A little later expression were seen in those vulval cells that are closest to the anchor cell and are beginning to invaginate. As vulval morphogenesis continues all of the cells that invaginate to form the vulva are expressing GFP. During this period, the uterine epithelium closest to the invaginating vulval cells begins to express cdh-3::GFP and the anchor cell fuses with the multinucleate uterine seam cell (utse), which also begins to express cdh-3::GFP. Expression continues in these cells into the adult stage, though at somewhat reduced levels, which may perhaps be due to perdurance of the fusion protein, since older adults show much reduced fluorescence compared with younger ones. During the time that the vulva is forming, cdh-3::GFP expressed in the six VC neurons located in the ventral nerve cord and the two HSNs located just posterior and dorsal to the developing vulva. These cells begin to extend processes at about this time, and GFP expression continues in these cells and their processes throughout the remainder of larval development and into adulthood.
  Picture: WBPicture0000007953
  Remark: Reporter gene fusion type not specified.
  Reference: WBPaper00002636
  Transgene: WBTransgene00027590