WormBase Tree Display for Expr_pattern: Expr1606
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| Expr1606 | Expression_of | Gene | WBGene00001599 | |
|---|---|---|---|---|
| WBGene00001598 | ||||
| Reflects_endogenous_expression_of | WBGene00001598 | |||
| WBGene00001599 | ||||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| GO_term | GO:0043186 | |||
| Subcellular_localization | Affinity-purified antibodies to either GLH-1 or GLH-2 proteins react with germ-line-specific P granules. P granules, as visualized by monoclonal antibodies directed against unidentified epitopes, are present in germ cells of all developmental stages with the exception of mature sperm. Anti-GLH-1 and anti-GLH-2 stain the same granules recognized by the anti-P-granule monoclonal antibodies; the granules are cytoplasmic in the oocyte and early embryo and perinuclear in later stage embryos. In adult worms, anti-GLH-1 brightly stains perinuclear P granules throughout the germ line of hermaphrodites and males. Anti-GLH-2 shows a more restricted pattern of staining that corresponds to the RNA distribution seen in situ; staining is less intense in the distal region than in the meiotic region of hermaphrodites and is barely detectable in the male germ line. P-granule staining is not detected with any preimmune sera or yolk. | |||
| Type | Antibody | Mouse polyclonal antibody against a fusion protein derived from GLH-1, and chicken polyclonal antibody against synthetic peptides corresponding to each N terminus, the region that is least conserved between GLH-1 and GLH-2. | ||
| Picture | WBPicture0000011620 | |||
| WBPicture0000011621 | ||||
| Reference | WBPaper00002611 | |||
| Antibody_info | WBAntibody00000135 | |||
| WBAntibody00000136 | ||||
| WBAntibody00000137 |
