WormBase Tree Display for Expr_pattern: Expr1600
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| Expr1600 | Expression_of | Gene | WBGene00000414 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000414 | ||
| Expression_data | Life_stage | WBls:0000005 | |
| WBls:0000010 | |||
| GO_term | GO:0005737 | ||
| Subcellular_localization | cytoplasm | ||
| Type | In_situ | Whole-mount in situ hybridization experiments using single-stranded digoxigenin-labeled antisense DNA probes made from the cec-1 cDNA. | |
| Pattern | Large amounts of cec-1 mRNA are localized in the cytoplasm of all blastomeres from early embryos. Indeed, cytoplasmic staining in the gonads of the hermaphrodites confirmed that maternal cec-1 mRNA is packaged into the oocytes. The signals were very strong in the early embryos prior to the onset of protein production at the 50- to 80-cell stage and weakened as development proceeded. Staining of the cytoplasm appeared uniform and no distribution gradient of cec-1 mRNA could be observed at any stage. | ||
| Remark | Hybridization of the embryos with the sense probe as control gave no signal. | ||
| Reference | WBPaper00002566 |
