WormBase Tree Display for Expr_pattern: Expr1599
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| Expr1599 | Expression_of | Gene | WBGene00000414 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000414 | |||
| Expression_data | Life_stage | WBls:0000022 | ||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0004017 | Certain | ||
| GO_term | GO:0005634 | |||
| GO:0005694 | ||||
| Subcellular_localization | CEC-1 staining in interphase nuclei was homogeneous and no speckles or foci could be detected, suggesting a uniform distribution of the protein. High-resolution imaging of the anti-CEC-1 antibody staining also revealed uniform staining of interphase nuclei. Interestingly, when the chromosomes started to condense during early mitosis, the protein appeared to dissociate from them, as shown in prometaphase nuclei. In fully condensed metaphase chromosomes, the protein was no longer associated with them. | |||
| Type | Antibody | Rabbit polyclonal antibody against CEC-1 recombinant protein. | ||
| Pattern | No CEC-1 could be detected in zygotes and early embryos. The protein was first detected in embryos at about the 50- to 80-cell stage. At this stage, weak immunostaining appeared in all somatic nuclei. Again, the specificity of the antibodies was confirmed by preincubating the serum with the purified cec-1-antigen which abolished all observed signals. Later in development, nuclear staining becomes stronger and persists in all somatic cells of wild-type and transgenic embryos and larvae. In adult wild-type worms, CEC-1 is present in all somatic nuclei. The intensity of the signals varied with the size of the nuclei; the large polyploid nuclei of the hypodermis and intestine showed intense immunofluorescence, while the smaller diploid nuclei had weaker signals. Neither in wild-type nor in transgenic animals were detected any nuclear staining in the germ line cells, in the germ precursor cells Z2 and Z3, and in early blastomeres. Furthermore, the presence of CEC-1 in the cytoplasm of these cells was excluded by comparison with control animals treated with the preimmune serum. | |||
| Reference | WBPaper00002566 | |||
| Antibody_info | WBAntibody00000123 |
