WormBase Tree Display for Expr_pattern: Expr13334
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Expr13334 | Expression_of | Gene | WBGene00007955 | ||
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Reflects_endogenous_expression_of | WBGene00007955 | ||||
Expression_data | Life_stage | WBls:0000002 | Anatomy_term (22) | ||
Anatomy_term (22) | |||||
GO_term | GO:0005783 | ||||
Not_in_Anatomy_term | WBbt:0005175 | Life_stage | WBls:0000002 | ||
WBbt:0005772 | Life_stage | WBls:0000002 | |||
Not_in_GO_term | GO:0005783 | ||||
Subcellular_localization | The translational Pselt-1.1::selt-1.1::gfp reporter revealed perinuclear localization, consistent with the ER localization previously reported for mammalian SELENOT. The ER localization of SELT-1.1 was confirmed by expressing into the QW1266[Pselt- 1.1::selt-1.1::gfp] the ER marker tram-1 fused to mcherry in muscle cells. | ||||
Type | Reporter_gene | ||||
Pattern | In hermaphrodites and males, selt-1.1 GFP expression was observed in neurons, epithelial and muscle cells of transgenic animals carrying both transcriptional (i.e. selt-1.2 promoter driving GFP expression) and translational (i.e. containing selt-1.2 promoter, exons and introns driving GFP expression) constructs. Crosses with the pan-neuronal marker rab-3 fused to the red fluorescent protein (RFP) in the nuclei confirmed that SELT-1.1 is expressed in all neurons of the nervous system. The expression of selt-1.1 in the ADL, ASH, ASI, ASJ, ASK, AWB amphid sensilla neurons was also confirmed by DiI staining. In the epithelia, selt-1.1 is expressed in the hypodermal, arcade, pharyngeal, vulval and rectal cells. selt-1.1 was not found to be expressed in the intestine or in the gonad. Muscle cells expressing selt-1.1 include the somatic muscle cells from head, neck and body wall as well as the non-striated pharyngeal muscles. SELT-1.1 expression was observed throughout development, from pre-bean embryonic stages to the adult stage. Embryos expressed GFP in most cells, also with a perinuclear localization. | ||||
Reference | WBPaper00051003 | ||||
Transgene | WBTransgene00032094 | ||||
WBTransgene00032095 |