WormBase Tree Display for Expr_pattern: Expr13301

Expr13301 Expression_of: Gene: WBGene00016313
    Reflects_endogenous_expression_of: WBGene00016313
  Expression_data: Life_stage: WBls:0000003 Anatomy_term: WBbt:0004017
    Anatomy_term: WBbt:0004017 Certain
        Life_stage: WBls:0000003
        Remark: Expressed broadly in embryos.
      WBbt:0005394 Certain
      WBbt:0006751 Certain
      WBbt:0006759 Certain
  Type: Reporter_gene
  Pattern: set-4p::GFP transgenic animals expressed GFP broadly in embryos. Post-embryonically, we detected fluorescence predominantly in the head and tail regions of the animal, in a pattern consistent with neuronal expression. To confirm this, we established a transgenic strain that co-expressed set-4p::GFP and mCherry driven by the pan-neuronal rab-3 promoter (Nonet et al., 1997). At all developmental stages interrogated, we observed co-localization of green and red fluorescence, consistent with somatic set-4p::GFP expression being predominantly neuronal. We did not observe significant GFP expression in intestine, body wall muscle, or hypodermis. The extent of co-localization of green and red fluorescence in set- 4p::GFP transgenic animals exposed to DiI reveals that the set-4 promoter is active in amphid sensory neurons as well as in other cells.
  Reference: WBPaper00050801
  Transgene: WBTransgene00032088