WormBase Tree Display for Expr_pattern: Expr1209
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| Expr1209 | Expression_of | Gene | WBGene00003588 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003588 | |||
| Expression_data | Anatomy_term | WBbt:0003681 | Certain | |
| WBbt:0005733 | Certain | |||
| WBbt:0006756 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | nuclei | |||
| Type | Reporter_gene | |||
| Pattern | The GFP is diffusely localized throughout the gland cell bodies within the terminal bulb of the pharynx, immediately anterior to the autofluorescent yellow granules that fill the cells of the intestine. When the vector nex-1-GFP 2 was used, punctate staining was also seen representing the nuclei of the two g1 gland cells of the terminal bulb. GFP staining was diffuse throughout the volume of the cell. Fluorescence of the spermathecal valve was also marked in nematodes expressing nex-1-GFP 2. The spermathecal valve is a single cell with four nuclei that is the product of the fusion of four cells. A region of nex-1 expression that was identified by the nex-1-GFP markers but was not identified by immunolocalization is the hypodermal cells. | |||
| Picture | WBPicture0000011696 | |||
| WBPicture0000011697 | ||||
| WBPicture0000011698 | ||||
| WBPicture0000011699 | ||||
| Remark | Identical expression patterns were obtained with these plasmids, although there appeared to be more cytoplasmic staining with nex-1-GFP 1 while nex-1-GFP 2 led to more concentrated nuclear staining in the same cells. The significance of these differences is presently unknown since both plasmids should express identical proteins; however, the observations made with the two different plasmids were made at different times so uncontrolled differences, such as variance in trans-gene dosage, between the experiments may have contributed to this difference. See Expr1210 for antibody staining of this gene. | |||
| Reference | WBPaper00003602 | |||
| Transgene | WBTransgene00027412 |
