During the L1 and L2 stages, Plet-7::gfppest expression is detected primarily in the intestine, the pharynx, and some neurons. Beginning in the L3, Plet-7::gfppest expression is detected in seam and vulva precursor cells, as well as the body wall muscle, in addition to the tissues observed in L1/L2 stage animals. Plet-7::gfppest is expressed strongly in the vulva and seam during the L4.