WormBase Tree Display for Expr_pattern: Expr11963
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| Expr11963 | Expression_of | Gene | WBGene00003932 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003932 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000041 | ||||
| WBls:0000023 | ||||
| Anatomy_term | WBbt:0004292 | Certain | ||
| WBbt:0005319 | Certain | |||
| WBbt:0005342 | Certain | |||
| WBbt:0005798 | Certain | |||
| WBbt:0005813 | Certain | |||
| WBbt:0005821 | Certain | |||
| WBbt:0006865 | Certain | |||
| GO_term | GO:0031430 | |||
| GO:0045178 | ||||
| GO:0055120 | ||||
| GO:0042383 | ||||
| GO:0098609 | ||||
| Subcellular_localization | In adult hermaphrodites, PAT-6::GFP is also located in the attachments of other muscles, including the uterine, anal depressor, anal sphincter, and vulval muscles, as well as in the spermatheca and the distal tip cells. | |||
| In wild-type 500 min embryos, PAT-6/actopaxin is polarized and located in nascent attachment complexes within the basal sarcolemma. | ||||
| PAT-6/actopaxin is first detectable in the myoblasts of early embryos, where it is distributed throughout the cytoplasm. When the embryos reach the 1.5-fold stage of development (430 min postfertilization), most of the PAT-6/actopaxin is polarized within the muscle cells and has entered nascent attachments. In each body wall muscle quadrant,PAT-6/actopaxin appears as a single longitudinal stripe of tightly clustered foci. These attachments have not yet moved into a regular striated array, and distinguishable dense body and M lines are not yet visible. PAT-6::GFP continues to colocalize with integrin at the body wall muscle attachments during the remaining stages of embryogenesis, larval development, and adulthood. PAT-6::GFP is found in adult dense bodies, M lines, and muscle-muscle adhesion plaques. Functional PAT-6/actopaxin::Cyan Fluorescent Protein (CFP) and PAT-3/integrin::Yellow Fluorescent Protein (YFP) fusion proteins expressed from a transgenic extrachomosomal array colocalize extensively in adult muscle attachments. | ||||
| Type | Antibody | |||
| Pattern | The anti-PAT-6/actopaxin antibody stains body wall muscle cell attachments of wild-type and pat-6 embryos that have been rescued by a functional pat-6::Green Fluorescent Protein (GFP) transgene. | |||
| Picture | WBPicture0000013332 | |||
| WBPicture0000013333 | ||||
| Reference | WBPaper00005915 | |||
| Transgene | WBTransgene00020328 | |||
| Antibody_info | WBAntibody00000630 |
