WormBase Tree Display for Expr_pattern: Expr1160
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| Expr1160 | Expression_of | Gene | WBGene00003783 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003783 | |||
| Expression_data | Life_stage | WBls:0000015 | ||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0004575 | Certain | ||
| WBbt:0004576 | Certain | |||
| Type | Antibody | Polyclonal antisera against NOS-1 fusion protein expressed in E. coli. | ||
| Pattern | NOS-1 protein is expressed sequentially during embryogenesis. No NOS-1 protein was detected in pregastrulation embryos, even though nos-1 RNA is present in these stages. NOS-1 first appeared in Z2 and Z3 at the 550-cell stage and remained expressed in these cells until the end of embryogenesis. | |||
| Picture | WBPicture0000008032 | |||
| Remark | The specificity of antibody was confirmed by staining embryos deficient for NOS-1. Affinity-purified anti-NOS-1 antibody detected NOS-1 in nos-2(RNAi) embryos but not in nos-1(RNAi) and nos-1(gv5) embryos. NOS-1 and NOS-2 thus do not depend on each other for expression. | |||
| To test whether NOS-1 is expressed zygotically, authors crossed nos-1(gv5) hermaphrodites (which express no NOS-1) to wild-type males and stained their progeny for NOS-1 protein. Detected NOS-1 expression in a pattern identical to that observed in embryos derived from wild-type hermaphrodites, confirming that NOS-1 expression is zygotic. | ||||
| Reference | WBPaper00003762 | |||
| Antibody_info | WBAntibody00000246 |
