WormBase Tree Display for Expr_pattern: Expr11508
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| Expr11508 | Expression_of | Gene | WBGene00013111 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00013111 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| WBls:0000023 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000013 | ||||
| WBls:0000032 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005135 | Certain | |||
| WBbt:0005212 | Certain | |||
| WBbt:0005735 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005813 | Certain | |||
| WBbt:0005829 | Certain | |||
| WBbt:0005830 | Certain | |||
| Type | Antibody | |||
| Reporter_gene | ||||
| Pattern | the sta-1 promoter::NLS::GFP reporter gene was widely expressed in a variety of cells and tissues, during most life stages. Specifically, it was expressed at higher levels in pharynx compared to expression levels in other tissues. GFP was also apparent in the entire intestine, marking all nuclei. Fluorescence intensities of GFP appeared weaker in the anterior intestine than in the posterior intestine, but this effect was due largely to the anterior part of the intestine being obscured by the gonad, where no reporter gene expression was observed. GFP expression was also observed in body muscles as well as in most of the nervous system. Among neuronal cells, GFP expression was readily observed in head ganglia, particularly in the posterior ganglia, including the small dorsal ganglion, two lateral ganglia, and ventral ganglion. Similarly, the sta-1 promoter was functional in the two lumbar ganglia, the dorsorectal ganglion of the tail, and the ventral nerve cord. Developmentally, reporter gene expression was first observed in enclosure stage embryos in a variety of cells, and expression persisted throughout embryogenesis, the four larval stages, and the entire adult life span. Neither cell- nor tissue-specific expression pattern changes were observed during development, nor was there any evident modulation of expression level, as judged by fluorescence intensity. Furthermore, GFP expression persisted throughout the dauer larval stage, although GFP expression level decreased significantly, particularly in the pharynx. Similar conclusions concerning expression pattern were suported by analysis of protein distribution by immunofluorescence. | |||
| Reference | WBPaper00028315 | |||
| Transgene | WBTransgene00031531 | |||
| Antibody_info | WBAntibody00001672 |
