WormBase Tree Display for Expr_pattern: Expr1093

Expr1093 Expression_of: Gene: WBGene00000214
    Reflects_endogenous_expression_of: WBGene00000214
  Expression_data: Life_stage: WBls:0000024
      WBls:0000027
      WBls:0000019
      WBls:0000020
      WBls:0000021
    Anatomy_term: WBbt:0005772 Certain
    GO_term: GO:0005737
      GO:0005764
      GO:0044444
  Subcellular_localization: Fluorescence signals were also present in the cytoplasm of the intestinal cells. The small, punctate structures that may represent protease-containing lysosomes were also observed. As observed in embryos, the protein is concentrated along the apical surface of the cell and outlines the intestinal lumen. It is also present in granules within the cytoplasm.
  Type: Antibody: Affinity-purified anti-ASP-1 IgGs.
  Pattern: Intense fluorescence signals disclosed that the protease is abundantly expressed at later stages of embryonic development. The protein is undetectable in embryos prior to the 2-fold stage of development. Affinity-purified anti-ASP-1 IgGs selectively bound to the apical surfaces of the intestinal cells in late embryos, thereby outlining the lumen of the gut. C. elegans larvae (L1L2) also contain substantial amounts of ASP-1, exclusively in the intestinal cells.
  Picture: WBPicture0000008147
  Remark: The results describing the cell- and development-specific patterns of asp-1 transcription, and mRNA and protein accumulation in embryonic and larval C. elegans, are all in excellent agreement.
  Reference: WBPaper00004299
  Antibody_info: WBAntibody00000301