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WormBase Tree Display for Expr_pattern: Expr10808

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Name Class

Expr10808Expression_ofGeneWBGene00000042
Reflects_endogenous_expression_ofWBGene00000042
Expression_dataAnatomy_termWBbt:0003679Certain
WBbt:0004991Certain
WBbt:0004993Certain
WBbt:0005274Certain
WBbt:0005278Certain
WBbt:0005336Certain
WBbt:0005339Certain
WBbt:0005821Certain
WBbt:0005829Certain
WBbt:0006749Certain
GO_termGO:0030425
GO:0043025
TypeAntibodyBecause live GFP fluorescence from oxSi39 was barely visible, the authors immunostained the animals using anti-GFP antibodies, rabbit anti-GFP (A11122; Invitrogen).
Reporter_gene
PatternACR-2::GFP showed mostly diffused localization on neuronal soma, as well as processes in the ventral cord, sublateral cords, and nerve ring. In the ventral cord, expression of ACR-2::GFP was specifically observed in the VA, VB, DA, and DB motor neurons as reported previously (Jospin et al., 2009). Several additional cells (e.g., SDQ, vulva muscles) also expressed ACR-2::GFP, presumably because of the use of a longer promoter and inclusion of intronic sequences. However, staining of ACR-2::GFP was not seen in the dorsal nerve cord. Because the axonal processes of DA and DB motor neurons are major constituents of the dorsal nerve cord, lack of ACR-2::GFP signals in the dorsal nerve cord indicates that ACR-2 is unlikely to localize to presynaptic sites of these motor neurons.
ReferenceWBPaper00042164
TransgeneWBTransgene00017768