WormBase Tree Display for Expr_pattern: Expr10808
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Expr10808 | Expression_of | Gene | WBGene00000042 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00000042 | ||
Expression_data | Anatomy_term (10) | ||
GO_term | GO:0030425 | ||
GO:0043025 | |||
Type | Antibody | Because live GFP fluorescence from oxSi39 was barely visible, the authors immunostained the animals using anti-GFP antibodies, rabbit anti-GFP (A11122; Invitrogen). | |
Reporter_gene | |||
Pattern | ACR-2::GFP showed mostly diffused localization on neuronal soma, as well as processes in the ventral cord, sublateral cords, and nerve ring. In the ventral cord, expression of ACR-2::GFP was specifically observed in the VA, VB, DA, and DB motor neurons as reported previously (Jospin et al., 2009). Several additional cells (e.g., SDQ, vulva muscles) also expressed ACR-2::GFP, presumably because of the use of a longer promoter and inclusion of intronic sequences. However, staining of ACR-2::GFP was not seen in the dorsal nerve cord. Because the axonal processes of DA and DB motor neurons are major constituents of the dorsal nerve cord, lack of ACR-2::GFP signals in the dorsal nerve cord indicates that ACR-2 is unlikely to localize to presynaptic sites of these motor neurons. | ||
Reference | WBPaper00042164 | ||
Transgene | WBTransgene00017768 |