WormBase Tree Display for Expr_pattern: Expr10060
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| Expr10060 | Expression_of | Gene | WBGene00000294 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000294 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000018 | ||||
| WBls:0000019 | ||||
| WBls:0000020 | ||||
| Anatomy_term | WBbt:0003679 | Certain | ||
| WBbt:0003681 | Certain | |||
| WBbt:0005319 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005813 | Certain | |||
| GO_term | GO:0030017 | |||
| GO:0031430 | ||||
| Subcellular_localization | In the adult body wall muscle, CAS-1 localized in a striated pattern indicating that it was associated with sarcomeres. Interestingly, CAS-1 only partially overlapped with bands of actin. Instead, strong striated staining of CAS-1 overlapped with MYO-3 that is enriched in the M-lines within the thick filaments. The enrichment of CAS-1 in the M-line was confirmed by colocalization with UNC-89, a specific marker of the M-lines. Relatively weakly stained spots of CAS-1 were present, but these did not co-localize with vinculin or alpha-actinin, indicating that CAS-1 did not localize to dense bodies, which are equivalent to the Z-discs in mammalian striated muscle. | |||
| Type | Antibody | |||
| Pattern | In embryos, CAS-1 was specifically expressed in the body wall muscle as early as the 1.5-fold stage (~420 min-old). CAS-1 was concentrated to a region where MYO-3 had been assembled into myofibrils. At the two-fold stage (~450-min old), CAS-1 remained associated with well-organized myofibrils, as indicated by the linear arrangement of MYO-3. Throughout the embryonic stages, expression of CAS-1 was specific in the body wall muscle, and no other embryonic tissues expressed detectable levels of CAS-1. In adult worms, CAS-1 was expressed in the body wall muscle, the pharynx, the neurons, the intestine, and the spermatheca. | |||
| Reference | WBPaper00041203 | |||
| Antibody_info | WBAntibody00002345 |
