WormBase Tree Display for Variation: WBVar02154211

WBVar02154211 Evidence: Curator_confirmed: WBPerson1983
  Name: Public_name: ve653
    Other_name: T20B3.2.1:c.-18_*4del
      T20B3.7.1:c.208+259_208+1604del
      T20B3.7.2:c.208+259_208+1604del
    HGVSg: CHROMOSOME_V:g.16833782_16835127del
  Sequence_details: SMap: S_parent: Sequence: T20B3
    Flanking_sequences: agcacataaaaatctacaaaaagatcacca cctggaaaagttgatctgtgagaagtggca
    Mapping_target: T20B3
    Type_of_mutation: Deletion
    SeqStatus: Sequenced
  Variation_type: Engineered_allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00049392
    Production_method: CRISPR_Cas9
    Status: Live
  Affects: Gene: WBGene00006585
      WBGene00004026
    Transcript: T20B3.2.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,start_lost,stop_retained_variant,5_prime_UTR_variant,3_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: T20B3.2.1:c.-18_*4del
        cDNA_position: 2-806
        Intron_number: 2-4/5
        Exon_number: 1-6/6
      T20B3.7.1 VEP_consequence: intron_variant
        VEP_impact: MODIFIER
        HGVSc: T20B3.7.1:c.208+259_208+1604del
        Intron_number: 3/7
      T20B3.7.2 VEP_consequence: intron_variant
        VEP_impact: MODIFIER
        HGVSc: T20B3.7.2:c.208+259_208+1604del
        Intron_number: 2/6
  Remark: Whole gene deletions made by the Rougvie, Moerman, Hutter and Sternberg labs that replace the coding sequence with a [LoxP + myo-2p::GFP::unc-54 3Prime UTR + rps-27p::neoR::unc-54 3Prime UTR + LoxP] cassette.
    Allele Description:tni-3(ve653[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) V.
    sgRNA1:GGAGGAAGAGGAATAAgaag sgRNA2: tttggcggggaaaaatgacc
  Method: Engineered_allele