WormBase Tree Display for Variation: WBVar00251951

WBVar00251951 Name: Public_name: tm3188
    HGVSg: CHROMOSOME_IV:g.12565714_12566230del
  Sequence_details: SMap: S_parent: Sequence: K08E7
    Flanking_sequences: tccagtatcaatacattttaggattgtttt gagattaaaagcaggaggcacaacatagta
    Mapping_target: K08E7
    Source_location: 7 CHROMOSOME_IV 12565713 12566231 Inferred_automatically: National_Bioresource_Project
    Type_of_mutation: Deletion
    PCR_product: tm3188_external
      tm3188_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Laboratory: FX
    Author: Mitani S
    DB_info: Database: National_Bioresource_Project seq 3188
    NBP_allele
    Status: Live
  Affects: Gene: WBGene00002002
      WBGene00010671
    Transcript: K08E7.2.3 VEP_consequence: 3_prime_UTR_variant
        VEP_impact: MODIFIER
        cDNA_position: 461-?
        Exon_number: 5/5
      K08E7.2.1 VEP_consequence: 3_prime_UTR_variant
        VEP_impact: MODIFIER
        cDNA_position: 461-?
        Exon_number: 5/5
      K08E7.2.2 VEP_consequence: 3_prime_UTR_variant
        VEP_impact: MODIFIER
        cDNA_position: 461-?
        Exon_number: 5/5
      K08E7.1.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        cDNA_position: ?-215
        CDS_position: ?-213
        Protein_position: ?-71
        Intron_number: 2/7
        Exon_number: 1-3/8
    Interactor: WBInteraction000051956
      WBInteraction000051958
      WBInteraction000504433
      WBInteraction000504435
      WBInteraction000504437
      WBInteraction000521008
      WBInteraction000534931
      WBInteraction000534932
      WBInteraction000534933
  Isolation: Mutagen: TMP/UV
  Genetics: Map: IV
  Description: Phenotype: WBPhenotype:0000061 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: eak-7 mutants live longer than wild-type animals Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000135 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: Mutants had increased DAF-16/FoxO target gene mRNA levels relative to wild-type animals. Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000308 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: eak-7 mutants undergo dauer arrest at 27C on plates lacking supplemental cholesterol, unlike wildtype Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001273 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: eak-7 mutants also exhibited resistance to heat stress Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001663 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: eak-7 mutants also exhibited resistance to oxidative stress Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001682 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: eak-7 mutants also exhibited resistance to ultraviolet stress Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000062 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson712
        Remark: Classified as homozygous viable by the National Bioresource Project of Japan. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson712
            Laboratory_evidence: FX
      WBPhenotype:0000114 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: Mutation of eak-7 did not significantly affect daf-16/FoxO mRNA levels Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000679 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: eak-7 mutant animals exhibited diffuse cytoplasmic fluorescence indistinguishable from that observed in wild-type animals Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000962 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: Mutations in eak-7 did not result in substantial changes in sod-3::GFP expression Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001400 Paper_evidence: WBPaper00036472
        Curator_confirmed: WBPerson2021
        Remark: Endogenous DAF-16/FoxO protein was undetectable in lysates from wild-type and eak-7 single mutants Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00036472
            Curator_confirmed: WBPerson2021
  Reference: WBPaper00036472
  Remark: 4705/4706-5222/5223 (517 bp deletion)
    This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence: WBPaper00041807
  Method: NBP_knockout_allele