WormBase Tree Display for Variation: WBVar00250733

WBVar00250733 Name: Public_name: tm1768
    Other_name: CE06191:p.Ile36_Ter128delinsLysLeuLeuLeuAlaLeu
      M05B5.5a.1:c.107_384delinsAGCTGTTGTTGGCTTTA
    HGVSg: CHROMOSOME_I:g.7193174_7193801delinsTAAAGCCAACAACAGCT
  Sequence_details: SMap: S_parent: Sequence: C01H6
    Flanking_sequences: aacaatcgctgatgtttctgcagaattcgt ttataaggataatcatatgcatttggaagc
    Mapping_target: C01H6
    Source_location: 7 CHROMOSOME_I 7193173 7193804 Inferred_automatically: National_Bioresource_Project
    Type_of_mutation: Insertion: TAAAGCCAACAACAGCTTA
      Deletion
    PCR_product: tm1768_external
      tm1768_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00022642
    Laboratory: FX
    Author: Mitani S
    DB_info: Database: National_Bioresource_Project seq 1768
    NBP_allele
    Status: Live
  Affects (3)
  Isolation: Mutagen: TMP/UV
  Genetics: Map: I
  Description: Phenotype: WBPhenotype:0000184 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson48
        Remark: Comment from Dr. B. Conradt to the National Bioresource Project of Japan: 3% NSM sister cell survival at 20C. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson48
            Laboratory_evidence: MD
        Penetrance: Low: Person_evidence: WBPerson7743
              Curator_confirmed: WBPerson48
          Range: 3 3 Person_evidence: WBPerson7743
                Curator_confirmed: WBPerson48
        EQ_annotations: Anatomy_term: WBbt:0003666 PATO:0000460 Person_evidence: WBPerson7743
                Curator_confirmed: WBPerson48
      WBPhenotype:0000188 Paper_evidence: WBPaper00033102
        Curator_confirmed: WBPerson2021
        Remark: Gonadal arms were short Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00033102
          Curator_confirmed: WBPerson2021
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00033102
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000396 Paper_evidence: WBPaper00033102
        Curator_confirmed: WBPerson2021
        Remark: Gonad arms were not reflexed in most gonads Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00033102
          Curator_confirmed: WBPerson2021
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00033102
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000684 Paper_evidence: WBPaper00033102
        Curator_confirmed: WBPerson2021
        Remark: A dramatic reduction in germ cell number was observed when hlh-2(tm1768) mutants were shifted to 25 C Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00033102
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: DAPI staining Paper_evidence: WBPaper00033102
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000688 Paper_evidence: WBPaper00039950
        Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson48
          WBPerson712
        Remark (2)
        Temperature_sensitive: Heat_sensitive: 25 Paper_evidence: WBPaper00039950
              Person_evidence: WBPerson7743
              Curator_confirmed: WBPerson48
                WBPerson712
      WBPhenotype:0000690 Paper_evidence: WBPaper00033102
        Curator_confirmed: WBPerson2021
        Remark: This allele exhibited recessive defects in hermaphrodite gonad arm migration (data not shown) Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00033102
          Curator_confirmed: WBPerson2021
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00033102
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000893 Paper_evidence: WBPaper00033102
        Curator_confirmed: WBPerson2021
        Remark: The hlh-2(tm1768) males had a significantly smaller germline mitotic region than controls Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00033102
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001652 Paper_evidence: WBPaper00039950
        Curator_confirmed: WBPerson712
        Remark: Animals exhibit a significant, 5%, defect in AC invasion at the P6.p four-cell stage. hlh-2(tm1768) animals with zero or299 two ACs were not observed. Defect is not stronger at higher temperatures. Paper_evidence: WBPaper00039950
            Curator_confirmed: WBPerson712
        Penetrance: Low: Paper_evidence: WBPaper00039950
              Curator_confirmed: WBPerson712
      WBPhenotype:0001656 Paper_evidence: WBPaper00033102
        Curator_confirmed: WBPerson2021
        Remark: Weakly dominant defects in hermaphrodite DTC specification. hlh-2 (tm1768 +RNAi) mutants at 25C show more severe DTC loss in hermaphrodites and linker cell loss in males as indicated by lag-2 and ceh-22 reporters Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        Semi_dominant: Paper_evidence: WBPaper00033102
          Curator_confirmed: WBPerson2021
        Variation_effect: Hypomorph_reduction_of_function: Paper_evidence: WBPaper00033102
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000057 PATO:0000460 Paper_evidence: WBPaper00033102
                Curator_confirmed: WBPerson2021
            WBls:0000056 PATO:0000460 Paper_evidence: WBPaper00033102
                Curator_confirmed: WBPerson2021
        Temperature_sensitive: Heat_sensitive: 25C Paper_evidence: WBPaper00033102
              Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: qIs57[lag-2::GFP] , qIs56[lag-2::GFP], qIs90[ceh- 22b::VENUS] Paper_evidence: WBPaper00033102
              Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000062 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson48
        Remark: Classified as homozygous viable by the National Bioresource Project of Japan. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson48
            Laboratory_evidence: FX
  Reference: WBPaper00039950
    WBPaper00033102
  Remark (2)
  Method: NBP_knockout_allele