WormBase Tree Display for Variation: WBVar00091925

WBVar00091925 Name: Public_name: ok641
    Other_name: R13F6.4a.2:c.1174_3204del
      CE47066:p.His727_Ser1403delextTer?
      CE42908:p.His574_Ser1250delextTer?
      CE46838:p.His392_Ser1068delextTer?
      R13F6.4f.1:c.1957_3987del
      R13F6.4d.1:c.1720_3750del
      R13F6.4a.1:c.1174_3204del
      R13F6.4e.1:c.2179_4209del
      CE46882:p.His653_Ser1329delextTer?
    HGVSg: CHROMOSOME_III:g.6839859_6841988del
  Sequence_details: SMap: S_parent: Sequence: R13F6
    Flanking_sequences: gattgttcagtttttgctgatgcaattgta agtcttgaaccacaagattctcgaaacaac
    Mapping_target: R13F6
    Type_of_mutation: Deletion
    PCR_product: ok641_external
      ok641_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00035839
    Laboratory: RB
    Person: WBPerson46
    KO_consortium_allele
    Status: Live
  Affects: Gene: WBGene00006498
    Transcript: R13F6.4f.1 (11)
      R13F6.4e.1 (11)
      R13F6.4a.1 (11)
      R13F6.4a.2 (11)
      R13F6.4d.1 (11)
    Interactor: WBInteraction000500418
      WBInteraction000500419
      WBInteraction000504386
  Isolation: Mutagen: UV/TMP
  Description: Phenotype: WBPhenotype:0000038 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: Some ten-1 homozygotes burst through the vulva due to germ cell leakage in the middle of the gonad Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000059 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: Ten-1 null mutants also arrest as L1 larvae Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000023 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
      WBPhenotype:0000174 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: As the gonadal precursor cells divided, a discontinuity appeared in the ten-1(ok641) gonadal BM that could be seen by a lack of laminin as well as of collagen IV LET-2 Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0005756 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Basement membrane was examined using LAM-1::GFP and LET-2 antibody Paper_evidence: WBPaper00032026
              Curator_confirmed: WBPerson2021
          Genotype: urEx131[LAM-1::GFP] Paper_evidence: WBPaper00032026
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000490 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: Ten-1 null mutants exhibit malformed pharynges and disorganized pharyngeal basement membranes Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0003681 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
          Life_stage: WBls:0000023 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: urEx131[LAM-1::GFP] Paper_evidence: WBPaper00032026
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000667 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: Z1 and Z4 cells were often displaced from the tips of the early L1 gonads in ten-1(ok641) worms. In almost 20% of the mutants, Z1 and/or Z4 cells interdigitated between germ cell precursors Z2 and Z3, or sometimes one of the SGPs was lost Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: qIs19 [lag-2::gfp] Paper_evidence: WBPaper00032026
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000688 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: Mutation of the ten-1 gene leads to sterility Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000041 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
      WBPhenotype:0000913 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: Basement Membrane was absent on the dorsal side of the broken gonad in ten-1 mutants and germ cells invaded the intestine Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0005756 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
            WBbt:0005175 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Basement membrane was examined via EM Paper_evidence: WBPaper00032026
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000977 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: Gonadal primordium of ten-1(ok641) larvae are ruptured. Germ cells are released into the body cavity and localize in the vicinity of the developing somatic gonad primordium. Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0005785 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0001215 Paper_evidence: WBPaper00032026
        Curator_confirmed: WBPerson2021
        Remark: There is no germline overproliferation in the early gonads of the ten-1(ok641) mutant Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00032026
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0005175 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
          Life_stage: WBls:0000023 PATO:0000460 Paper_evidence: WBPaper00032026
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: pie-1p::GFP::PGL-1 Paper_evidence: WBPaper00032026
              Curator_confirmed: WBPerson2021
  Reference: WBPaper00032026
    WBPaper00025910
  Remark: Sequenced by the C. elegans Gene Knockout Consortium Paper_evidence: WBPaper00041807
  Method: KO_consortium_allele