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WormBase Tree Display for Variation: WBVar00091001

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Name Class

WBVar00091001EvidencePaper_evidenceWBPaper00026975
NamePublic_namene2257
Other_nameCE00315:p.Ile173Phe
T05G5.3.1:c.517A>T
HGVSgCHROMOSOME_III:g.9748088A>T
Sequence_detailsSMapS_parentSequenceT05G5
Flanking_sequencesgccgatttcggacttgccagagctattggttcccgattcgcgtttacacgcatgaagtga
Mapping_targetT05G5
Type_of_mutationSubstitutionatPaper_evidenceWBPaper00026975
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00040431
LaboratoryWM
StatusLive
AffectsGeneWBGene00000405
TranscriptT05G5.3.1 (12)
InteractorWBInteraction000502269
WBInteraction000519779
WBInteraction000524640
WBInteraction000524644
WBInteraction000525090
WBInteraction000538741
WBInteraction000538742
GeneticsInterpolated_map_positionIII0.968216
DescriptionPhenotypeWBPhenotype:0000822Paper_evidenceWBPaper00036019
Curator_confirmedWBPerson712
RemarkMales exhibit gonadal feminization.Paper_evidenceWBPaper00036019
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitivePaper_evidenceWBPaper00036019
Curator_confirmedWBPerson712
WBPhenotype:0001133Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
RemarkThe EMS cell underwent an aberrant left/right division, instead of a wild type anterior/posterior division (Table 1)Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
PenetranceHigh95Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006876PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Life_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
GO_termGO:0051301PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBPhenotype:0001276Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
RemarkFigure S2 legend: "Instead of localizing to the single germline blastomere present in 16-cell stage embryos [S3], PIE-1 protein is mislocalized in somatic blastomeres in cdk-1(ne2257) and gsk-3(RNAi) embryos."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004873PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Life_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Phenotype_assayGenotypePIE-1::GFPPaper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBPhenotype:0001404Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
RemarkFigure S2 legend: "SKN-1 protein levels persist at high levels in the C-lineage of cdk-1(ne2257) and gsk-3(RNAi) embryos at the 12-cell stage, while SKN-1 staining is not observed in wild-type at this stage [S2]."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0003810PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBbt:0006875PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Life_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBPhenotype:0001636Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
RemarkAnimals exhibited C-derived gut (Table 1)Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
PenetranceIncomplete36Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0003810PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Life_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
GO_termGO:0048565PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Phenotype_assayTreatment"C-derived gut specification was followed in laser-operated embryos."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBPhenotype:0001645Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Remark"In wild-type animals, OMA-1 and its homolog OMA-2 accumulate during oogenesis and remain high until the 1-cell stage, but rapidly decline during the first and second mitosis [9, 10] (Figure 2 and Figure S3). We examined the timing of OMA-1 degradation by monitoring the expression of an OMA-1::GFP protein. We found that OMA-1 protein levels remain high in gsk-3, cdk-1, cks-1, and mbk-2 mutant embryos (Figure 2, Figure S3, and data not shown)."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
"In par-1 mutants, GFP::ZF1 protein is expressed uniformly in all blastomeres until the 2-cell stage but is degraded rapidly when the embryo divides from two to four cells. This degradation is dependent on ZIF-1. We found that in gsk-3(RNAi), cdk-1(ne2257), and oma-1(ne411gf) mutants, the GFP::ZF1 signal remains high from the 4- to 8-cell stage (Figure 6A), suggesting that stabilized OMA-1 interferes with ZIF-1-dependent proteolysis."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
EQ_annotationsLife_stageWBls:0000003PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
GO_termGO:0006508PATO:0000460Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeOMA-1::GFPPaper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
par-1(RNAi)Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000354Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Remark"In contrast, cdk-1(ne236), cdk-1(ne2257), and cks-1(ne549) homozygotes have no obvious larval phenotypes and produce mutant embryos with normal cell divisions and well-differentiated cells and tissues (Figure S1, data not shown)."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBPhenotype:0000746Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Remark"In contrast, cdk-1(ne236), cdk-1(ne2257), and cks-1(ne549) homozygotes have no obvious larval phenotypes and produce mutant embryos with normal cell divisions and well-differentiated cells and tissues (Figure S1, data not shown)."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBPhenotype:0000750Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Remark"In contrast, cdk-1(ne236), cdk-1(ne2257), and cks-1(ne549) homozygotes have no obvious larval phenotypes and produce mutant embryos with normal cell divisions and well-differentiated cells and tissues (Figure S1, data not shown)."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBPhenotype:0001370Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Remark"We found that the CDK-1 protein encoded by the ne2257 mutant allele, CDK-1(I173F), binds to CKS-1, CYB-1, and CYB-3 as efficiently as does wild-type CDK-1 (Figures 4A, 4B, and 4E) and that both cyclin complexes recovered from cdk-1(ne2257) mutant extracts exhibit near wild-type activity toward histone H1 (Figures 4A and 4B)."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
WBPhenotype:0002213Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
Remark"We found that the CDK-1 protein encoded by the ne2257 mutant allele, CDK-1(I173F), binds to CKS-1, CYB-1, and CYB-3 as efficiently as does wild-type CDK-1 (Figures 4A, 4B, and 4E) and that both cyclin complexes recovered from cdk-1(ne2257) mutant extracts exhibit near wild-type activity toward histone H1 (Figures 4A and 4B)."Paper_evidenceWBPaper00026975
Curator_confirmedWBPerson2987
ReferenceWBPaper00036019
WBPaper00026975
WBPaper00065825
MethodSubstitution_allele