WormBase Tree Display for Variation: WBVar00144965
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WBVar00144965 | Evidence | Paper_evidence | WBPaper00026735 | |||||
---|---|---|---|---|---|---|---|---|
Name | Public_name | e2740 | ||||||
Sequence_details | SeqStatus | Pending_curation | ||||||
Variation_type | Allele | |||||||
Origin | Species | Caenorhabditis elegans | ||||||
Strain | WBStrain00004664 | |||||||
Laboratory | CB | |||||||
Status | Live | |||||||
Affects | Gene | WBGene00010442 | ||||||
Isolation | Mutagen | mut-7 | ||||||
Description | Phenotype | WBPhenotype:0001014 | Person_evidence | WBPerson261 | ||||
Curator_confirmed | WBPerson48 | |||||||
Remark | Resistant to infection by M. nematophilum. | Person_evidence | WBPerson261 | |||||
Curator_confirmed | WBPerson48 | |||||||
Penetrance | Complete | Person_evidence | WBPerson261 | |||||
Curator_confirmed | WBPerson48 | |||||||
Recessive | Person_evidence | WBPerson261 | ||||||
Curator_confirmed | WBPerson48 | |||||||
Variation_effect | Loss_of_function_undetermined_extent | Person_evidence | WBPerson261 | |||||
Curator_confirmed | WBPerson48 | |||||||
WBPhenotype:0001411 | Paper_evidence | WBPaper00026735 | ||||||
Curator_confirmed | WBPerson48 | |||||||
Remark | Based on lectin binding properties to the cuticle | Paper_evidence | WBPaper00026735 | |||||
Curator_confirmed | WBPerson48 | |||||||
Phenotype_assay | Treatment | Tested the following fluorescein or rhodamine-conjugated lectins: WGA, SBA, TPA, PEA,, CON, LEA. | Paper_evidence | WBPaper00026735 | ||||
Curator_confirmed | WBPerson48 | |||||||
WBPhenotype:0001413 | Paper_evidence | WBPaper00026735 | ||||||
WBPaper00037686 | ||||||||
Curator_confirmed | WBPerson48 | |||||||
WBPerson712 | ||||||||
Remark | Slight tail swelling observed when grown with M. nematophilum. | Paper_evidence | WBPaper00026735 | |||||
Curator_confirmed | WBPerson48 | |||||||
Phenotype is perceptively weaker than br5, and significant rectal accumulation of the M. nematophilum pathogen can be seen in some infected worms. | Paper_evidence | WBPaper00037686 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Penetrance | Incomplete | variable | Paper_evidence | WBPaper00026735 | ||||
Curator_confirmed | WBPerson48 | |||||||
WBPhenotype:0001415 | Paper_evidence | WBPaper00026735 | ||||||
Curator_confirmed | WBPerson48 | |||||||
Remark | Based on vital dye staining for bacteria to reveal M. nematophilum; less rectal staining, indicating less adherence in the usual region, but some abnormal staining in males relative to wild type. | Paper_evidence | WBPaper00026735 | |||||
Curator_confirmed | WBPerson48 | |||||||
WBPhenotype:0001420 | Paper_evidence | WBPaper00029153 | ||||||
WBPaper00031925 | ||||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Leaky Bah - biofilm formed on the side of the head and not over the mouth, allowing normal feeding and growth. | Paper_evidence | WBPaper00029153 | |||||
Curator_confirmed | WBPerson712 | |||||||
Animals are completely resistant to attachment of biofilm produced by Xenorhabdus nematophila. | Paper_evidence | WBPaper00031925 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Penetrance | Incomplete | Phenotype is somewhat leaky. | Paper_evidence | WBPaper00029153 | ||||
Curator_confirmed | WBPerson712 | |||||||
Complete | Paper_evidence | WBPaper00031925 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | Biofilm formation on adult worms was assessed for worms incubated 24-48 hours on the bacterial lawn. Alternatively, gravid adults were allowed to lay eggs on bacterial lawns for several hours, then removed and larvae were assessed for biofilm attachment the next day. | Paper_evidence | WBPaper00031925 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0002109 | Paper_evidence | WBPaper00062695 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | We used a bus-12(e2740) that has a fragile cuticle (Darby et al. 2007) and found DEC had similar effects compared to the N2 (Fig 1C and D). The IC50 for bus-12 was 0.50.04mM, indistinguishable from N2, indicating DEC passes through the cuticle, and its lack of potency may be due to reduced effect on its target receptor. | Paper_evidence | WBPaper00062695 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00003038 | Paper_evidence | WBPaper00062695 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBMol:00007968 | Paper_evidence | WBPaper00062695 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_not_observed | WBPhenotype:0001209 | Paper_evidence | WBPaper00029153 | |||||
Curator_confirmed | WBPerson712 | |||||||
Penetrance | Incomplete | Phenotype is somewhat leaky. | Paper_evidence | WBPaper00029153 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001212 | Paper_evidence | WBPaper00026735 | ||||||
Curator_confirmed | WBPerson48 | |||||||
Remark | Based on a comparison of bleach sensitivity with wild type worms. | Paper_evidence | WBPaper00026735 | |||||
Curator_confirmed | WBPerson48 | |||||||
Reference | WBPaper00026735 | |||||||
WBPaper00031925 | ||||||||
WBPaper00029153 | ||||||||
WBPaper00037686 | ||||||||
WBPaper00062695 | ||||||||
Remark | mut-7 induced | |||||||
Method | Allele |