WormBase Tree Display for Transgene: WBTransgene00006263
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WBTransgene00006263 | Public_name | mgEx701 | |||||
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Summary | [mlt-10(mg364); sur-5::GFP] | ||||||
Synonym | [mlt-10(mg364)] | ||||||
Construction | Construct | WBCnstr00006117 | |||||
Construction_summary | The array mgEx701 was made by microinjection of mlt-10(mg364) (5 ng/?l) DNA, along with a sur-5::gfp plasmid (50 ng/?l) (Yochem et al., 1998) and pBS (40 ng/?l) into wild-type adults. To ameliorate the toxicity associated with increased dosage of mlt-10, transgenic nematodes were cultured on bacteria expressing mlt-10 dsRNA. (Meli et al. 2010). | ||||||
Genetic_information | Extrachromosomal | ||||||
Phenotype | WBPhenotype:0000638 | Paper_evidence | WBPaper00036066 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Remark | mgEx701 rendered wild-type larvae unable to shed old cuticles | Paper_evidence | WBPaper00036066 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Caused_by_gene | WBGene00015646 | Paper_evidence | WBPaper00036066 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Reference | WBPaper00036066 | ||||||
Species | Caenorhabditis elegans |