WormBase Tree Display for Transgene: WBTransgene00005878

WBTransgene00005878 Public_name: WBPaper00034765Ex1
  Summary: [snb-1p:: G85R hSOD1]
  Construction: Construct: WBCnstr00005746
  Construction_summary: A PCR-amplified genomic DNA segment extending from minus 3021 bp to just upstream of the SNB start codon, was inserted in place of the unc-54 enhancer/promoter in the plasmid pPD30_38, and the various human SOD cDNA-containing segments were then adjoined.
  Genetic_information: Extrachromosomal
    Phenotype: WBPhenotype:0000017 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: G85R animals were strongly resistant to aldicarb as compared with the corresponding wild-type animals Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00003650 Paper_evidence: WBPaper00034765
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000039 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: Reduced survival Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000154 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: Reduced brood size Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000273 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: Side-to-side thrashing movement in liquid was severely affected in G85R SOD transgenic animals Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000568 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: Ventral nerve cord processes show striking morphologic abnormalities that include reduced diameter and reduced numbers of organelles within them Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000569 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: Some of the VNC processes in G85R animals were swollen by large vesicular structures Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000571 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: Reduced numbers of vesicles within VNC processes Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000616 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: The G85R mutant animals exhibited a discontinuous pattern of synaptobrevin positive puncta in the dorsal nerve cord. In addition to the reduced number of puncta, their signals were also reduced. Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: nuIs152 [Punc-129::GFP-snb-1] Paper_evidence: WBPaper00034765
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000633 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: The number of VNC processes (mostly axons) in G85R animals was slightly reduced compared with wild-type Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000847 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: When the presynaptic active zone protein UNC-10 (RIM1) was examined, it exhibited a diminished number of puncta in G85R animals Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001405 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: Aggregates formed in the unfused G85R animals exhibited a more diffuse, fluffy character. Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001670 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: A decrease in number and vesicle content in the dorsal cord was observed via the fluorescent-tagged synaptic vesicle reporters YFP-RAB-3 and synapsin-1-YFP. EM analysis shows a diminished number of presynaptic vesicles. Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: nuIs168 [Punc- 129::YFP-Rab-3], nuIs163 [Punc-129::snn-1-YFP] Paper_evidence: WBPaper00034765
              Curator_confirmed: WBPerson2021
      WBPhenotype:0004025 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: G85R SOD transgenic animals exhibited minimal forward movement across the culture medium compared to control Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000845 Paper_evidence: WBPaper00034765
        Curator_confirmed: WBPerson2021
        Remark: G85R animals displayed the same sensitivity to levamisole-induced paralysis as wild-type Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Caused_by_other: Human SOD1 Paper_evidence: WBPaper00034765
            Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00004019 Paper_evidence: WBPaper00034765
              Curator_confirmed: WBPerson2021
  Disease_info: Models_disease: DOID:332
    Models_disease_in_annotation: WBDOannot00000796
  Reference: WBPaper00034765
  Species: Caenorhabditis elegans