WormBase Tree Display for Transgene: WBTransgene00005525
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| WBTransgene00005525 | Public_name | otIs176 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Summary | [dpy-7::hst-6] | ||||||||
| Construction | Construct | WBCnstr00005444 | |||||||
| Genetic_information | Integrated | ||||||||
| Phenotype | WBPhenotype:0000426 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Remark | Increased staining with an antibody that recognizes HS epitopes is observed consistently in the hypodermis upon misexpression of hst-6 in this tissue. | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Caused_by_gene | WBGene00002031 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Phenotype_assay | Treatment | Immunostaining with the AO4B08 HS-specific antibody | Paper_evidence | WBPaper00032413 | |||||
| Curator_confirmed | WBPerson2021 | ||||||||
| WBPhenotype:0000727 | Paper_evidence | WBPaper00032413 | |||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Remark | Biochemical analyses of HS from transgenic worms expressing hst-6 in the hypodermis display an increase of 6O-sulfated relative to 2O-sulfated HS disaccharides | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Caused_by_gene | WBGene00002031 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| WBPhenotype:0001767 | Paper_evidence | WBPaper00032413 | |||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Remark | The transgenic line shows a significant number of animals in which the projections of individual DA and DB motor neurons are rerouted | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Caused_by_gene | WBGene00002031 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0005278 | PATO:0000460 | Paper_evidence | WBPaper00032413 | ||||
| Curator_confirmed | WBPerson2021 | ||||||||
| WBbt:0005274 | PATO:0000460 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Phenotype_not_observed | WBPhenotype:0000306 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Remark | Expression patterns of slt-1 and sax-3 reporters appear unchanged in animals with ectopic hst-6 expression | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Caused_by_gene | WBGene00002031 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Phenotype_assay | Genotype | kyIs174 [slt-1::gfp], kyEx251[sax-3::gfp] | Paper_evidence | WBPaper00032413 | |||||
| Curator_confirmed | WBPerson2021 | ||||||||
| WBPhenotype:0000701 | Paper_evidence | WBPaper00032413 | |||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Remark | Hypodermal cell fate, as visualized by normal AJM-1 expression and localization patterns, is not visibly affected by ectopic hst-6 expression | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Caused_by_gene | WBGene00002031 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Phenotype_assay | Genotype | ajm-1::GFP | Paper_evidence | WBPaper00032413 | |||||
| Curator_confirmed | WBPerson2021 | ||||||||
| WBPhenotype:0000703 | Paper_evidence | WBPaper00032413 | |||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Remark | Hypodermal cell morphology, as visualized by normal AJM-1 expression and localization patterns, is not visibly affected by ectopic hst-6 expression | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Caused_by_gene | WBGene00002031 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Phenotype_assay | Genotype | ajm-1::GFP | Paper_evidence | WBPaper00032413 | |||||
| Curator_confirmed | WBPerson2021 | ||||||||
| WBPhenotype:0001399 | Paper_evidence | WBPaper00032413 | |||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Remark | Basement membrane topology, as visualized by a lam-1::gfp reporter, is not visibly affected by ectopic hst-6 expression | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Caused_by_gene | WBGene00002031 | Paper_evidence | WBPaper00032413 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Phenotype_assay | Genotype | lam-1::gfp | Paper_evidence | WBPaper00032413 | |||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Reference | WBPaper00032413 | ||||||||
| Species | Caenorhabditis elegans |
