WormBase Tree Display for Transgene: WBTransgene00004683

WBTransgene00004683 Public_name: syIs63
  Summary: [cog-1::GFP + unc-119(+)]
  Synonym: [cog-1::cog-1-gfp]
  Construction: Construct: WBCnstr00004665
    Integration_method: Gamma_irradiation
  Construction_summary: There are three cog-1::gfp fusions made. These fusions insert the gfp coding region into three different positions of pBP150: the 5' end of exon 1; a unique SmaI site in exon 1; and the 3' end of cog-1. The construct with the insertion in the SmaI site was able to rescue the mutant phenotype of sy275. The construct with the 5' insertion did not rescue either mutant allele. The reason for this is not clear. The two exon 1 constructs should label only the cog-1A transcript; however, no obvious difference in the expression pattern was observed between these and the 3' construct. The following describes the expression pattern of an integrated transgenic array syIs63, which contains the 5' insert, unless noted otherwise. To make the 5' construct, PCR amplified gfp coding region was cloned into the AatII site of pBP150, placing the GFP 17 amino acids downstream of the first ATG of cog-1.
  Genetic_information: Integrated
  Used_for: Expr_pattern: Expr2276
      Expr14269
      Expr15875
      Expr1170047
      Marker114
    Marker_for: PDA cell Paper_evidence: WBPaper00031556
      PDA neuron Paper_evidence: WBPaper00031556
    Interactor: WBInteraction000502882
      WBInteraction000502883
      WBInteraction000502884
      WBInteraction000502885
  Associated_with: Strain: WBStrain00029528
      WBStrain00030904
      WBStrain00030922
      WBStrain00030960
  Reference: WBPaper00005610
    WBPaper00006052
    WBPaper00006260
    WBPaper00024291
    WBPaper00025135
    WBPaper00026718
    WBPaper00027031
    WBPaper00028375
    WBPaper00029327
    WBPaper00029390
    WBPaper00031349
    WBPaper00031556
    WBPaper00040151
    WBPaper00040503
    WBPaper00040964
    WBPaper00041116
    WBPaper00042542
    WBPaper00045660
    WBPaper00048387
    WBPaper00048898
    WBPaper00050164
    WBPaper00054757
    WBPaper00056194
    WBPaper00058673
    WBPaper00062065
  Species: Caenorhabditis elegans