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WormBase Tree Display for Strain: WBStrain00055386

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Name Class

WBStrain00055386Genotyperab-39(sy1987) II.
Public_namePS9934
ContainsGeneWBGene00004286
VariationWBVar02159123
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received02 Aug 2023 00:00:00
LocationCGC
RemarkSuperficially wild type. CRISPR/Cas9 engineered STOP-IN null mutant of rab-39. Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). Left flanking sequence: CTACCAATATCGTCTTATCGTTATTGGGGATTCAAC. Right flanking sequence: AGTTGGCAAATCTAGTCTTTTACGGTACTTCACAG. Inserted sequence between the two-flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: GTTATTGGGGATTCAACAGT. Method Reference: Wang H, et al. G3 (Bethesda). 2018 Nov 6;8(11):3607-3616.Inferred_automaticallyFrom CGC strain data
Made_by: Heenam ParkCGC_data_submission
SpeciesCaenorhabditis elegans