WormBase Tree Display for Strain: WBStrain00052771
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WBStrain00052771 | Status | Live | ||
---|---|---|---|---|
Genotype | vit-2(crg9070[vit-2::gfp]) vit-1(hq503[vit-1::mCherry]) X. | |||
Public_name | MQD2798 | |||
Contains | Gene | WBGene00006925 | ||
WBGene00006926 | ||||
Variation | WBVar02158569 | |||
Properties | Outcrossed | x6 | ||
Mutagen | CRISPR_Cas9 | |||
CGC_received | 23 Mar 2023 00:00:00 | |||
Location | MQD | |||
CGC | ||||
Remark | [2023-04-25T18:29:28.667Z WBPerson51134] New Strain: Genotype vit-1(hq503 [vit-1::mCherry]) vit-2(crg9070[vit-2::gfp]) X | Curator_confirmed | WBPerson51134 | |
mCherry knocked into C terminal of vit-1 by CRISPR/Cas9 in the background of parental strain BCN9071 vit-2(crg9070[vit-2::gfp]) X. This resulting double-labelled strain was crossed six times with N2 to remove potential off-target mutations. mCherry and GFP are co-localized in the intestine, body cavity, oocyte, and embryo in adult hermaphrodites. Reference: Zhai C, et al. Aging cell, 21(11), e13719. https: | ||||
Made_by: Chao Zhai | CGC_data_submission | |||
Species | Caenorhabditis elegans |