WormBase Tree Display for Strain: WBStrain00051874
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| WBStrain00051874 | Status | Live | |
|---|---|---|---|
| Genotype | nhr-49(et8) I; mdt-15(et14) III. | ||
| Public_name | QC155 | ||
| Contains | Gene | WBGene00003639 | |
| WBGene00007016 | |||
| Variation | WBVar02125064 | ||
| WBVar02125060 | |||
| Properties | Outcrossed | x10 | |
| Mutagen | EMS | ||
| CGC_received | 16 Sep 2021 00:00:00 | ||
| Location | CGC | ||
| Made_by | WBPerson11984 | ||
| Remark | This double mutant strain contains an excess polyunsaturated fatty acids in its cell membranes accompanied by excess lipid peroxidation, cell permeability, increased autophagy and other defects. The nhr-49(et8) C9873765T [WS200] mutation can be detected by PCR using the following primers: nhr-49 Fwd: 5-CAGATTATGATTCGTGATGCTAGA-3; nhr-49 WT Rev: 5-GAGATGAAAGATGTTGCTGTAGAG-3; nhr-49 Mut Rev: 5-GAGATGAAAGATGTTGCTGTAGAA-3. Annealing 65C, expected products ~300 bp. The mdt-15(et14) C5832666T [WS200] mutation can be detected by PCR using the following primers: mdt-15(et14) Mut Fwd: 5-GTGCCTCCAGATCCACAGCT-3; mdt-15(et14) WT Fwd: 5-GTGCCTCCAGATCCACAGCC-3; mdt-15 Rev: 5-CACCCATTGGAGCACCACT-3. Annealing 65C, expected product ~400 bp. Reference: Devkota R, et al. Genetics (in press). Volume 219, Issue 1, September 2021. https: | ||
| Species | Caenorhabditis elegans |
