WormBase Tree Display for Strain: WBStrain00049816
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| WBStrain00049816 | Status | Live | ||
|---|---|---|---|---|
| Genotype | acs-13(et54) nhr-49(et8) I; mdt-15(et14) III. | |||
| Public_name | QC156 | |||
| Contains | Gene | WBGene00003639 | ||
| WBGene00007016 | ||||
| WBGene00022037 | ||||
| Variation | WBVar02156930 | |||
| WBVar02125064 | ||||
| WBVar02125060 | ||||
| Properties | Outcrossed | x10 | ||
| Mutagen | EMS | |||
| CGC_received | 16 Sep 2021 00:00:00 | |||
| Location | QC | |||
| CGC | ||||
| Made_by | WBPerson11984 | |||
| Remark | [2021-12-19T00:03:31.583Z WBPerson51134] New Strain: acs-13(et54) | Curator_confirmed | WBPerson51134 | |
| This triple mutant strain contains an excess polyunsaturated fatty acids in its cell membranes accompanied by excess lipid peroxidation, cell permeability, increased autophagy and other defects. The acs-13(et54) mutation (G125R) can be detected using PCR with the following primers: WT FWD: 5CTA CCA GGG TGT TCG CCA TG 3; acs-13 mutant FWD: 5CTA CCA GGG TGT TCG CCA TA 3; acs-13 REV: 5TCA AAC TTG GGC ATT GCT CC 3. Annealing 65C, expected product 395 bp. The nhr-49(et8) C9873765T [WS200] mutation can be detected by PCR using the following primers: nhr-49 Fwd: 5-CAGATTATGATTCGTGATGCTAGA-3; nhr-49 WT Rev: 5-GAGATGAAAGATGTTGCTGTAGAG-3; nhr-49 Mut Rev: 5-GAGATGAAAGATGTTGCTGTAGAA-3. Annealing 65C, expected products ~300 bp. The mdt-15(et14) C5832666T [WS200] mutation can be detected by PCR using the following primers: mdt-15(et14) Mut Fwd: 5-GTGCCTCCAGATCCACAGCT-3; mdt-15(et14) WT Fwd: 5-GTGCCTCCAGATCCACAGCC-3; mdt-15 Rev: 5-CACCCATTGGAGCACCACT-3. Annealing 65C, expected product ~400 bp. Reference: Devkota R, et al. Genetics (in press). Volume 219, Issue 1, September 2021. https: | ||||
| Species | Caenorhabditis elegans |
