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WormBase Tree Display for Strain: WBStrain00037581

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Name Class

WBStrain00037581StatusLive
GenotypeF29B9.2(ok3628) IV/nT1 [qIs51] (IV;V).
Public_nameVC2878
ContainsGeneWBGene00017920
VariationWBVar00278409
RearrangementnT1
TransgeneWBTransgene00001903
PropertiesOutcrossedx1
MutagenEMS
CGC_received25 May 2010 00:00:00
LocationCGC
Made_byWBPerson14471
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
F29B9.2. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3628 homozygotes (probable embryonic arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GCAGCTTATCAAGCACACGA. External right primer: AACCGCGTGAAAGAATATGG. Internal left primer: CATCTCCGGACACCACTACA. Internal right primer: GCTTCTTCATGAGGCGATCT. Internal WT amplicon: 1193 bp. Deletion size: 823 bp. Deletion left flank: ATCAAGGAAGGTCAGACACTGCTCATCCCG. Deletion right flank: ACTATTGTGGATCCCATCTCGAAGCACGCG.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans