WormBase Tree Display for Strain: WBStrain00000297
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| WBStrain00000297 | Status | Live | ||
|---|---|---|---|---|
| Genotype | nbs-1(me102) meIs8/mIn1 [mIs14 dpy-10(e128)] II. | |||
| Public_name | AV828 | |||
| Contains | Gene | WBGene00001072 | ||
| WBGene00007504 | ||||
| Variation | WBVar00142982 | |||
| WBVar02149633 | ||||
| Rearrangement | mIn1 | |||
| Transgene | WBTransgene00000999 | |||
| WBTransgene00014904 | ||||
| Properties | Outcrossed | x4 | ||
| Mutagen | EMS | |||
| CGC_received | 28 Dec 2018 00:00:00 | |||
| Location | CGC | |||
| Remark | meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. Transgene contains a combination of cDNA and genomic sequences of cosa-1 including 212 bp of 3'UTR. GFP is expressed in the adult germline as 6 bright foci per nucleus (one per chromosome pair) from late pachytene through diplotene stages. Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP me103 homozygotes (sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. nbs-1(me103) homozygotes have frayed and aggregated chromosomes at diakinesis of meiosis I. References: Girard C, et al. Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4443-E4452. Yokoo R, et al. Cell. 2012 Mar 30;149(1):75-87. | Inferred_automatically | From CGC strain data | |
| Made_by: Chloe Girard, Baptiste Roelens, Karl A. Zawadzki | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
