WormBase Tree Display for RNAi: WBRNAi00108063

WBRNAi00108063 Homol: Homol_homol: ZK973:RNAi
  Sequence_info: PCR_product: sjj_ZK973.k
  Experiment: Genotype: daf-2(e1370); akt-1(ok525)
    Temperature: 19
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: ZK973.3 Inferred_automatically: RNAi_primary
    Gene: WBGene00022834 Inferred_automatically: RNAi_primary
      WBGene00022832 Inferred_automatically: RNAi_primary
    Transcript: ZK973.3.1 Inferred_automatically: RNAi_primary
      ZK973.8.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Interaction: WBInteraction000536031
  Reference: WBPaper00038374
  Phenotype: WBPhenotype:0000637 Remark: "Using dauer formation as the readout, we performed genetic epistasis experiments to identify the substrate of PDP-1. We first tested whether pdp-1 acted directly through the IIS pathway by focusing on kinase mutants downstream of daf-2 (Table 1 and Figure S9). pdk-1(sa680), daf-2(e1370); akt-1(ok525) and daf-2(e1370); akt-2(ok393) mutants were maintained on vector, daf-18 and pdp-1 RNAi and dauer formation of these strains was assayed at the appropriate temperatures. Interestingly, pdp-1 RNAi resulted in suppression of dauer formation of pdk-1(sa680) mutants, daf-2(e1370); akt-1(ok525) and daf-2(e1370); akt-2(ok393) worms (Table 1 and Figure S9)."
  Remark: (Table 1, Figure S9) pdp-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
  Method: RNAi