WormBase Tree Display for RNAi: WBRNAi00107838

WBRNAi00107838 Homol: Homol_homol: B0336:RNAi
  Sequence_info: PCR_product: sjj_B0336.8
  Experiment: Genotype: UNC-119::mCherry; gld-1(RNAi)
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: B0336.8 Inferred_automatically: RNAi_primary
    Gene: WBGene00002982 Inferred_automatically: RNAi_primary
    Transcript: B0336.8.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00049105
  Phenotype: WBPhenotype:0000354 Remark: "To assess neuronal differentiation, the tumor was induced in the pan-neuronal marker (UNC-119::mCherry) strain by gld-1 RNAi and maximum z-projections covering whole gonad arms were used to score the area occupied by UNC-119::mCherry-expressing cells. Concomitant downregulation of either lgg-3 or atg-7 -- both required for autophagy (Fig 3C) -- leads to a reduction of the area occupied by UNC-119::mCherry-expressing cells in the tumor by around 50% and 30%, respectively (Fig 4B and C). Therefore, depletion of autophagy results in impairment of neuronal differentiation within the germline tumor."
      EQ_annotations: Anatomy_term: WBbt:0006796 PATO:0000460
        GO_term: GO:0030154 PATO:0000460
  Remark: (Figure 4B,4C) lgg-3 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
  Method: RNAi