WormBase Tree Display for RNAi: WBRNAi00103054

WBRNAi00103054 Homol: Homol_homol: Y39A1C:RNAi
  Sequence_info: PCR_product: sjj_Y39A1C.2
  Experiment: Date: 03 Oct 2012 00:00:00
    Genotype: unc-119(ed4)III; hhIs64 [unc-119(?); sur-5::UbV-GFP]III (strain listed as PP563).
    Treatment: Animals grown on 0.5 or 1 mM paraquat.
    Temperature: 20
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: Y39A1C.2 Inferred_automatically: RNAi_primary
    Gene: WBGene00003898 Inferred_automatically: RNAi_primary
    Transcript: Y39A1C.2.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00041777
  Phenotype: WBPhenotype:0000460 Remark: Knockdown of oxi-1 by RNAi only minimally affected the degradation of UbV-GFP under basal conditions. However exposing worms to low doses of the oxidative stress-inducing agent paraquat resulted in stabilization of UbV-GFP (loss of degradation).
      Affected_by: Molecule: WBMol:00002747
    WBPhenotype:0001645 Remark: Knockdown of oxi-1 by RNAi only minimally affected the degradation of UbV-GFP under basal conditions. However exposing worms to low doses of the oxidative stress-inducing agent paraquat resulted in stabilization of UbV-GFP (loss of degradation).
      Affected_by: Molecule: WBMol:00002747
  Remark: Authors engineered a ubiquitin fusion degradation(UFD) substrate in which a noncleavable ubiquitin is N-terminally fused to GFP (UbV-GFP) and expressed under the control of the sur-5 promoter to monitor protein degradation in vivo.
  Method: RNAi