WormBase Tree Display for RNAi: WBRNAi00102808

WBRNAi00102808 Homol: Homol_homol: B0336:RNAi
  Sequence_info: PCR_product: sjj_B0336.8
  Experiment: Laboratory: FE
    Date: 16 Jun 2013 00:00:00
    Strain: WBStrain00005592
    Treatment: The level of autophagy was investigated using an LGG-1::GFP translational reporter. GFP positive puncta/seem cell were counted in L4 transgenic worms at 1000X magnification using a Leica DMI 6000 B microscope. Counting puncta is usually performed in L3 animals as autofluorescence increases with age and obscures LGG-1::GFP visualization. We investigated the level of autophagy in young L4 worms in order to age-match the assay with the feeding and pumping rate assays. All animals were kept at 20 degrees Celsius and assayed by the same experimenter. The number of autophagosomes per seem cell was averaged for each worm and this average was used for calculating the mean number of LGG-1::GFP containing puncta/seam cell. Statistical analysis was performed by unpaired two-tailed t-test (with Welch's correction if variances were significantly different). The Bonferroni method was used to correct for multiple comparisons and P-values equivalent to a significance level of 0.05 were considered statistically significant. N used for analysis is the total number of worms observed for each treatment.
    Temperature: 20
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: B0336.8 Inferred_automatically: RNAi_primary
    Gene: WBGene00002982 Inferred_automatically: RNAi_primary
    Transcript: B0336.8.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Interaction: WBInteraction000525082
  Reference: WBPaper00043981
  Phenotype: WBPhenotype:0000679 Remark: atg-12(RNAi) resulted in reduced numbers of LGG-1::GFP puncta, suggesting reduced levels of autophagy
    WBPhenotype:0002283 Remark: atg-12(RNAi) resulted in reduced numbers of LGG-1::GFP puncta, suggesting reduced levels of autophagy
  Remark: (Figure 2A) atg-12/lgg-3 RNAi
  Method: RNAi