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WormBase Tree Display for RNAi: WBRNAi00102023

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Name Class

WBRNAi00102023HomolHomol_homolC09B8:RNAi
Sequence_infoPCR_productsjj_C09B8.7
ExperimentLaboratoryKU
Date04 Dec 2009 00:00:00
Genotypemax-2(nv162)
TreatmentFor pak-1 RNAi by injection, the double-stranded RNA for pak-1 was prepared by in vitro transcription. Worms from the L4 larval stage to the young adult stage were injected with the double-stranded RNA for pak-1 and then allowed to lay eggs on NGM plates. Embryos were transferred to NGM plates containing 100 micromolar copper sulfate.
Delivered_byInjection
InhibitsPredicted_geneC09B8.7bInferred_automaticallyRNAi_primary
C09B8.7cInferred_automaticallyRNAi_primary
C09B8.7aInferred_automaticallyRNAi_primary
GeneWBGene00003911Inferred_automaticallyRNAi_primary
TranscriptC09B8.7c.1Inferred_automaticallyRNAi_primary
C09B8.7b.1Inferred_automaticallyRNAi_primary
C09B8.7a.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00035583
Phenotype_not_observedWBPhenotype:0000593RemarkInjection of the double-stranded pak-1 RNA did not enhance the copper sensitivity of max-2(nv162) mutants.
Affected_byMoleculeWBMol:00002862
Remarkpak-1 RNAi (Figure 1G)
MethodRNAi