WormBase Tree Display for RNAi: WBRNAi00101802

WBRNAi00101802 Homol: Homol_homol: W10C8:RNAi
  Sequence_info: PCR_product: sjj_W10C8.2
  Experiment: Laboratory: LW
    Date: 04 May 2009 00:00:00
    Genotype: rrf-3(pk1426) II; ayIs6(hlh-8::gfp) X
    Treatment: To observe the effects of RNAi during postembryonic development, RNAi was performed by ingestion with a synchronous population of L1 larvae. L1 animals synchronized by standard methods were plated in triplicate on HT115(DE3) bacteria expressing dsRNA for genes of interest. Bacteria for ingestion were prepared using 4 mM IPTG to induce dsRNA production. RNAi-P was performed at 25 degrees Celsius and animals were scored for M lineage phenotypes or used for immunostaining 24-48 h after plating.
    Temperature: 25
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: W10C8.2 Inferred_automatically: RNAi_primary
    Gene: WBGene00004077 Inferred_automatically: RNAi_primary
    Transcript: W10C8.2.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00033137
  Phenotype: WBPhenotype:0000095 Remark: Postembryonic pop-1(RNAi) resulted in animals with both fewer sex myoblasts (SMs) derived from the M-lineage (in 41.4% of animals) as well extra sex myoblasts (SMs) derived from the M-lineage (in 9.1% of animals), as determined by hlh-8::GFP. Authors report that the loss of SMs was due to a fate transformation of M.v(l/r)pa to the fate of its posterior sister M.d(l/r)pp (normally fated to become body wall muscles (BWMs)).
      Penetrance: Range: 9
    WBPhenotype:0000414 Remark: Postembryonic pop-1(RNAi) resulted in animals with both fewer sex myoblasts (SMs) derived from the M-lineage (in 41.4% of animals) as well extra sex myoblasts (SMs) derived from the M-lineage (in 9.1% of animals), as determined by hlh-8::GFP. Authors report that the loss of SMs was due to a fate transformation of M.v(l/r)pa to the fate of its posterior sister M.d(l/r)pp (normally fated to become body wall muscles (BWMs)).
      Penetrance: Range: 41
    WBPhenotype:0000961 Remark: Postembryonic pop-1(RNAi) resulted in animals with both fewer sex myoblasts (SMs) derived from the M-lineage (in 41.4% of animals) as well extra sex myoblasts (SMs) derived from the M-lineage (in 9.1% of animals), as determined by hlh-8::GFP. Authors report that the loss of SMs was due to a fate transformation of M.v(l/r)pa to the fate of its posterior sister M.d(l/r)pp (normally fated to become body wall muscles (BWMs)).
      Penetrance: Range: 9
    WBPhenotype:0002191 Remark: Postembryonic pop-1(RNAi) resulted in animals with both fewer sex myoblasts (SMs) derived from the M-lineage (in 41.4% of animals) as well extra sex myoblasts (SMs) derived from the M-lineage (in 9.1% of animals), as determined by hlh-8::GFP. Authors report that the loss of SMs was due to a fate transformation of M.v(l/r)pa to the fate of its posterior sister M.d(l/r)pp (normally fated to become body wall muscles (BWMs)).
      Penetrance: Range: 41
    WBPhenotype:0002192 Remark: Postembryonic pop-1(RNAi) resulted in animals with both fewer sex myoblasts (SMs) derived from the M-lineage (in 41.4% of animals) as well extra sex myoblasts (SMs) derived from the M-lineage (in 9.1% of animals), as determined by hlh-8::GFP. Authors report that the loss of SMs was due to a fate transformation of M.v(l/r)pa to the fate of its posterior sister M.d(l/r)pp (normally fated to become body wall muscles (BWMs)).
      Penetrance: Range: 9
  Remark: (Figure S4, Table 2) pop-1 RNAi-P (postembryonic RNAi). Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
  Method: RNAi