WormBase Tree Display for RNAi: WBRNAi00101665
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| WBRNAi00101665 | Homol | Homol_homol | W10C8:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_W10C8.2 | |||
| Experiment | Laboratory | CT | |||
| Date | 01 Nov 2010 00:00:00 | ||||
| Genotype | wIs78 [SCMp::GFP marker transgene for seam cells]; hbl-1(mg285) | ||||
| Treatment | RNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | W10C8.2 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00004077 | Inferred_automatically | RNAi_primary | ||
| Transcript | W10C8.2.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00037838 | ||||
| Phenotype_not_observed | WBPhenotype:0000166 | Remark | RNAi did not affect the precocious seam cell fusion phenotype of hbl-1(mg285) mutants | ||
| Remark | (Figure 8A) pop-1 RNAi | ||||
| Method | RNAi |
